Ph.D.-thesis at the Max-Planck-Institut fuer molekulare Physiologie
Dortmund, Germany
Department of Physical Biochemistry
"Biochemical and Biophysical Studies on Bacteriorhodopsin"

Diploma (Diplomarbeit) at the Max-Planck-Institut fuer molekulare Physiologie
Dortmund
Department of Physical Biochemistry
"Synthesis of [3-13C]Cysteine and Biosynthetic Incorporation of Cysteine in T89C-Bacteriorhodopsin"

Lab work in the Department of Organic Chemistry
University Dortmund
"Photochemical-sensibilized radical intramolecular cyclization"

Lab work in the Department of Toxicology
Institut fuer Arbeitsphysiologie at the University Dortmund
"Gaschromatographic determination of ethylene and ethyleneoxid in car exhausts"

Used techniques:


Organic Synthesis:

Asymmetric synthesis of L-cysteine via chiral Ni2+ complex


Molecular biology:

Construction of template DNA (PCR, digestion, ligation, electroporation, plasmid purification)
Runoff transcription
Expression and isotope-labeling of membraneproteins (bacteriorhodopsin and rhodopsin) in bacteria (E. coli), archaea (H. salinarium), and mammalian cells (HEK 293)
Cell-free expression of bacterioopsin in E. coli extracts


Protein purification:

gelelectrophoresis
differential centrifugation
gelfiltration
ion exchange chromatography
immunoaffinity chromatography


Biophysical methods:

solid state NMR
FTIR