Steven O. Smith

Professional Honors

Woods Hole Oceanographic Fellowship, Woods Hole Oceanographic Institute, 1978.

Telonicher Fellowship, Department of Biology, California State University, Humboldt, 1979.

University of California Regents Fellowship, Department of Chemistry, University of California, Berkeley, 1983-1984.

NIH Postdoctoral Fellowship, Francis Bitter National Magnet Laboratory, Massachusetts Institute of Technology, 1985-1987.

Searle Scholars Award, 1988-1990.

Recent publications

Takeshi Sato, Payal Pallavi, Urszula Golebiewska, Stuart McLaughlin and Steven O. Smith Structure of the membrane reconstituted transmembrane-juxtamembrane peptide EGFR(622-660) and its interactions with Ca2+/Calmodulin. Biochemistry 2006;45, 12704-12714

Wenyi Zhang, Takeshi Sato and Steven O. Smith NMR spectroscopy of basic/aromatic amino acid clusters in membrane proteins. Progress in Nuclear Magnetic Resonance Spectroscopy 2006;48, 183-199

Judith Staerk, Catherine Lacout, Takeshi Sato, Steven O. Smith, William Vainchenker and Stefan N. Constantinescu An amphipathic motif at the transmembrane-cytoplasmic junction prevents autonomous activation of the thrombopoietin receptor. Blood 2006;107, 1864-1871

Takeshi Sato, Pascal Kienlen-Campard, Mahiuddin Ahmed, Wei Liu, Huilin Li, James I. Elliott, Saburo Aimoto, Stefan N. Constantinescu, Jean-Noel Octave and Steven O. Smith Inhibitors of amyloid toxicity based on B-sheet packing of AB40 and Ab42. Biochemistry 2006;45, 5503-5516

Christoph Seibert, Weiwen Ying, Svetlana Gavrilov, Fotini Tsamis, Shawn E. Kuhmann, Anandan Palani, Jayaram R. Tagat, John W. Clader, Stuart W. McCombie, Bahige M. Baroudy, Steven O. Smith, Tatjana Dragic, John P. Moore, Thomas P. Sakmar Interaction of small molecule inhibitors of HIV-1 entry with CCR5. Virology 2006;349, 41-54

Iris A. Mastrangelo, Mahiuddin Ahmed, Takeshi Sato, Wei Liu, Chengpu Wang, Paul Hough and Steven O. Smith High-resolution Atomic Force Microscopy of Soluble AB42 Oligomers. Journal of Molecular Biology 2006;358, 106-119

Evan Crocker, Markus Eilers, Shivani Ahuja, Viktor Hornak, Amiram Hirshfeld, Mordechai Sheves and Steven O. Smith Location of Trp265 in Metarhodopsin II: Implications for the Activation Mechanism of the Visual Receptor Rhodopsin. Journal of Molecular Biology 2006;357, 163-172

Lori L. Anderson, Garland R. Marshall, Evan Crocker, Steven O. Smith and Thomas J. Baranski Motion of Carboxyl Terminus of Ga Is Restricted upon G Protein Activation. A Solution NMR Study Using Semisynthetic Ga Subunits. The Journal of Biological Chemistry 2005;Vol. 280, No. 35, 31019-31025.

Wei Liu, Evan Crocker, Stefan N. Constantinescu, and Steven O. Smith Helix Packing and Orientation in the Transmembrane Dimer of gp55-P of the Spleen Focus Forming Virus. Biophysical Journal 2005;89(2), 1194-1202

Wenyi Zhang and Steven O. Smith Mechanism of Penetration of Antp(43-58) into Membrane Bilayers. Biochemistry 2005; Vol. 44, No. 30, 10110-10118

Stuart McLaughlin, Steven O. Smith, Micheal J. Hayman and Diana Murray An Electrostatic Engine Model for Autoinhibition and Activation of the Epidermal Growth Factor Receptor (EGFR/ErbB) Family. Journal of General Physiology 2005;Vol. 126, No. 1, 41-53.

Markus Eilers, Viktor Hornak, Steven O. Smith and James B. Konopka Comparison of Class A and D G Protein-Coupled Receptors: Common Features in Structure and Activation. Biochemistry 2005;Vol. 44, No. 25, 8959-8975.

Kathleen P. Howard, Wei Lui, Evan Crocker, Vikas Nanda, James Lear, William F. Degrado and Steven O. Smith Rotational Orientation of Monomers Within a Designed Homo-Oligomer Transmembrane Helical Bundle. Protein Science 2005;Vol. 14, No. 4, 1019-1024.

Katharina F. Kubatzky, Wei Liu, Kerri Goldgraben, Carlos Simmerling, Steven O. Smith and Stefan N. Constantinescu Structural Requirements of the Extracellular to Transmembrane Domain Junction for Erythropoietin Receptor Function. The Journal of Biological Chemistry 2005;Vol. 280, No. 15, 14844-14854.

Wei Liu, Evan Crocker, Wenyi Zhang, James I. Elliot, Burkhard Luy, Huilin Li, Saburo Aimoto and Steven O. Smith Structural Role of Glycine in Amyloid Fibrils Formed from Transmembrane a-Helices. Biochemistry 2005;44, 3591-3597

Ashish B. Patel, Evan Crocker, Philip J. Reeves, Elena V. Getmanova, Markus Eilers, H. Gobind Khorana and Steven O. Smith Changes in Interhelical Hydrogen Bonding upon Rhodopsin Activation. Journal of Molecular Biology 2005;347, 803-812

Ashish B. Patel, Evan Crocker, Markus Eilers, Amiram Hirshfeld, Mordechai Sheves and Steven O. Smith Coupling of retinal isomerization to the activation of rhodopsin. PNAS 2004;101, 10048-10053

Alok Gambhir, Gyöngyi Hangyás-Mihályné, Irina Zaitseva, David S. Cafiso, Jiyao Wang, Diana Murray, Srinivas N. Pentyala, Steven O. Smith and Stuart McLaughlin Electrostatic Sequestration of PIP2 on Phospholipid Membranes by Basic/Aromatic Regions of Proteins. Biophysical Journal 2004;86, 2188-2207

Crocker E, Patel AB, Eilers M, Jayaraman S, Getmanova E, Reeves PJ, Ziliox M, Khorana HG, Sheves M, and Steven O. Smith Dipolar assisted rotational resonance NMR of tryptophan and tyrosine in rhodopsin. Journal of Biomolecular NMR 2004;29(1), 11-20

Wei Liu, Markus Eilers, Ashish B. Patel, and Steven O. Smith Helix Packing Moments Reveal Diversity and Conservation in Membrane Protein Structure. Journal of Molecular Biology 2004;337(3), 713-729

Elena Getmanova, Ashish B. Patel, Judith Klein-Seetharaman, Michele C. Loewen, Philip J. Reeves, Noga Friedman, Mordechai Sheves, Steven O. Smith, and H. Gobind Khorana NMR Spectroscopy of Phosphorylated Wild-Type Rhodopsin: Mobility of the Phosphorylated C-Terminus of Rhodopsin in the Dark and upon Light Activation. Biochemistry 2004;43(4), 1126-1133

Nadine Seubert, Yohan Royer, Judith Staerk, Katharina F. Kubatzky, Virginie Moucadel, Shyam Krishnakumar, Steven O. Smith, and Stefan N. Constantinescu Active and Inactive Orientations of the Transmembrane and Cytosolic Domains of the Erythropoietin Receptor Dimer. Molecular Cell 2003; 12, 1239-1250

Zhang W, Crocker E, McLaughlin S, and Steven O. Smith Binding of Peptides with Basic and Aromatic Residues to Bilayer Membranes: phenylalanine in the myristoylated alanine-rich C kinase substrate effector domain penetrates into the hydrophobic core of the bilayer. J. Biol. Chem. 2003; 278(24), 21459-21466

Wei Liu, Evan Crocker, David J. Siminovitch, and Steven O. Smith Role of Side-Chain Conformational Entropy in Transmembrane Helix Dimerization of Glycophorin A. Biophysical Journal 2003;84(2), 1263-1271

Jennifer C. Lin, William Parrish, Markus Eilers, Steven O. Smith, and James B. Konopka Aromatic Residues at the Extracellular Ends of Transmembrane Domains 5 and 6 Promote Ligand Activation of the G Protein-Coupled a-Factor Receptor. Biochemistry 2003;42(2), 293-301

Eilers, M., Ying, W., Reeves, P.J., Khorana, H.G. and Smith, S.O. Magic angle spinning nuclear magnetic resonance of isotopically labeled rhodopsin. Methods Enzymol. 2002;343, 212-222

Pablo M. Irusta, Yue Luo, Omar Bakht, Char-Chang Lai, Steven O. Smith, and Daniel DiMaio Definition of an Inhibitory Juxtamembrane WW-like Domain in the Platelet-derived Growth Factor b Receptor. J Biol Chem 2002;277(41), 38627-38634

Brigitte Schobert, Jill Cupp-Vickery, Viktor Hornak, Steven O. Smith, Janos K. Lanyi Crystallographic Structure of the K Intermediate of Bacteriorhodopsin: Conservation of Free Energy after Photoisomerization of the Retinal. Journal of Molecular Biology 2002;321(4), 715-726

Steven O. Smith, Charles Smith, Srinivasan Shekar, Olve Peersen, Martine Ziliox, and, and Saburo Aimoto Transmembrane Interactions in the Activation of the Neu Receptor Tyrosine Kinase. Biochemistry 2002;41(30), 9321-9332

Markus Eilers, Ashish B. Patel, Wei Liu, and Steven O. Smith Comparison of Helix Interactions in Membrane and Soluble a-Bundle Proteins. Biophysical Journal 2002;82(5), 2720-2736

Steven O. Smith, Markus Eilers, David Song, Evan Crocker, Weiwen Ying, Michel Groesbeek, Guenter Metz, Martine Ziliox, and Saburo Aimoto Implications of Threonine Hydrogen Bonding in the Glycophorin A Transmembrane Helix Dimer. Biophysical Journal 2002;82(5), 2476-2486

Steven O. Smith, Toru Kawakami, Wei Liu, Martine Ziliox, and Saburo Aimoto Helical Structure of Phospholamban in Membrane Bilayers. Journal of Molecular Biology 2001;313(5), 1139-1148

Steven O. Smith, David Song, Srinivasan Shekar, Michel Groesbeek, Martine Ziliox, and Saburo Aimoto Structure of the Transmembrane Dimer Interface of Glycophorin A in Membrane Bilayers . Biochemistry 2001;40(22), 6553-6558

Weiwen Ying, Scott E. Irvine, Richard A. Beekman, David J. Siminovitch, and Steven O. Smith Deuterium NMR Reveals Helix Packing Interactions in Phospholamban. Journal of the American Chemical Society 2000; 122(45); 11125-1112

Dragic,T., Trkola, A., Thompson, D.A.D., Cormier, E.G., Kajumo, F.A., Maxwell, E., Lin, S.W., Ying, W., Smith, S.O., Sakmar, T.P. and Moore, J.P. A binding pocket for a small molecule inhibitor of HIV-1 entry within the transmembrane helices of CCR5.Proc. Natl. Acad. Sci. USA 97, 5639-5644 (2000)

Eilers, M., Shekar, S.C., Shieh, T., Smith, S.O. and Fleming, P.J. Internal packing of helical membrane proteins. Proc. Natl. Acad. Sci. USA 97, 5796-5801 (2000)

Zahn, T.J., Eilers, M., Guo, Z.M., Ksebati, M.B., Simon, M., Scholten, J.D., Smith, S.O. and Gibbs, R.A. Evaluation of isoprenoid conformation in solution and in the active site of protein-farnesyl transferase using carbon-13 labeling in conjunction with solution- and solid-state NMR. J. Am. Chem. Soc. 122, 7153-7164 (2000)

Constantinescu, S.N., Liu, X.D., Beyer, W., Fallon, A., Shekar, S., Henis, Y.I., Smith, S.O. and Lodish, H.F. Activation of the erythropoietin receptor by the gp55-P viral envelope protein is determined by a single amino acid EMBO J. 18, 3334-3347 (1999)

Eilers, M., Reeves, P.J., Ying, W., Khorana, H.G. and Smith, S.O. Magic angle spinning NMR of the protonated retinylidene Schiff base in rhodopsin: Expression of 15N-lysine- and 13C-glycine-labeled opsin in a stable cell line. Proc. Natl. Acad. Sci. USA 96, 487-492 (1999)

Klein, O., Kegler-Ebo, D., Su, J., Smith, S.O. and Dimaio, D. The bovine papillomavirus E5 protein requires a juxtamembrane negative charge for activation of the platelet-derived growth factor beta receptor and transformation of C127 cells.J. Virology 73, 3264-3272 (1999)

Javadpour, M.M.,Eilers, M., Groesbeek, M. and Smith, S.O.
Helix Packing in Polytopic Membrane Proteins: Role of Glycine in Transmembrane Helix Association. Biophys. J. 77, 1609-1618 (1999)

Reeves, P.J., Klein-Seetharaman, J., Getmanova, E.V., Eilers, M., Loewen, M.C., Smith, S.O. and Khorana, H.G.
Expression and purification of rhodopsin and its mutants from stable mammalian cell lines: application to NMR studies.
Biochem. Soc. Trans. 27, 950-955 (1999).

Han, M.,Groesbeek, M., Smith, S.O. and Sakmar, T.P. The role of the C9 methyl group in rhodopsin activation: characterization of mutant opsin with the artificial chromophore 11-cis-9-demethylretinal.Biochemistry 37, 538-545 (1998)

Han, M., Smith, S.O. and Sakmar, T.P. Constitutive activation of opsin by mutation of methionine 257 on transmembrane helix 6. Biochemistry 37, 8253-8261 (1998)

Klein, O., Polack, G.W., Surti, T., Kegler-Ebo, D., Smith, S.O. and Dimaio, D. Role of glutamine 17 of the bovine papillomavirus E5 protein in platelet-derived growth factor beta receptor activation and cell transformation. J. Virology 72, 8921-8932 (1998)

Surti, T., Klein, O., Aschheim, K., Dimaio, D. and Smith, S.O. Structural models of the bovine papillomavirus E5 protein. Proteins: Struct. Funct. Genet. 33, 601-612 (1998)

Arkin, I.T., Adams, P.D., Brunger, A.T., Aimoto, S., Engelman, D.M. and Smith, S.O. Structure of the transmembrane cysteine residues in phospholamban. J. Membr. Biol. 155, 199-206 (1997)

Arkin, I.T., Adams, P.D., Brunger, A.T., Smith, S.O. and Engelman, D.M. Structural perspectives of phospholamban, a helical transmembrane pentamer. Annu. Rev. Biophys. Biomol. Struct. 26, 157-179 (1997)

Groesbeek, M. and Smith, S.O. Synthesis of19-fluororetinal and 20-fluororetinal. J. Org. Chem. 62, 3638-3641 (1997)

Han, M., Groesbeek, M., Sakmar, T.P. and Smith, S.O. The C9 methyl group of retinal interacts with glycine-121 in rhodopsin. Proc. Natl. Acad. Sci. USA 94, 13442-13447 (1997)

Han, M., Lou, J.H., Nakanishi, K., Sakmar, T.P. and Smith, S.O. Partial agonist activity of 11-cis-retinal in rhodopsin mutants.J. Biol. Chem. 272, 23081-23085 (1997).

Petti, L.M., Reddy, V., Smith, S.O. and Dimaio, D. Identification of amino acids in the transmembrane and juxtamembrane domains of the platelet-derived growth factor receptor required for productive interaction with the bovine papillomavirus E5 protein. J. Virology 71, 7318-7327 (1997)

Sansom, M.S., Smith, G.R., Smart, O.S. and Smith, S.O. Channels formed by the transmembrane helix of phospholamban: a simulation study. Biophys. Chem. 69, 269-281 (1997)

Shieh, T., Han, M., Sakmar, T.P. and Smith, S.O. The steric trigger in rhodopsin activation. J. Mol. Biol. 269, 373-384 (1997)

Arkin, I.T., Mackenzie, K., Fisher, L., Aimoto, S., Engelman, D. and Smith, S.O. Mapping the lipid exposed surface of membrane proteins. Nature Struct. Biol. 3, 240-243 (1996)

Han, M., Lin, S., Smith, S.O. and Sakmar, T.P. The effects of amino acid replacements of glycine 121 on transmembrane helix 3 of rhodopsin. J. Biol. Chem. 271, 32330-32336 (1996)

Han, M., Lin, S., Minkova, M., Smith, S.O. and Sakmar, T.P. Functional helix-helix interactions in rhodopsin: replacement of phenylalanine 261 by alanine causes reversion of phenotype of a glycine 121 replacement mutant. J. Biol. Chem. 271, 32337-32342 (1996)

Ludlam, C. Arkin, I.T., Liu, X.-M., Rothman, M.S., Rath, P., Aimoto, S., Smith, S.O., Engelman, D. and Rothschild, K.J. FTIR spectroscopy and site-directed isotope labeling as a probe of local secondary structure in the transmembrane domain of phospholamban Biophys. J. 70, 1728-1736 (1996)

Metz, G., Ziliox, M. and Smith, S.O. Towards quantitative CP-MAS NMR Solid-State NMR. 7, 155-160 (1996)

Smith, S.O. NMR studies on the structure and function of rhodopsin. Behav. Brain Sci. 18, 488-489 (1996)

Sanders, C.R., Czerski, L., Vinogradova, O., Badola, P., Song, D. and Smith, S.O. E.coli diacylglycerol kinase is an a-helical polytopic membrane protein and can spontaneously insert into pre-formed lipid vesicles. Biochemistry 35, 8610-8618 (1996)

Smith, S.O., Smith, C.S. and Bormann, B.J. Strong hydrogen bonding interactions involving a buried glutamic acid in the transmembrane domain of the neu/erbB-2 receptor. Nature Struct. Biol. 3, 252-258 (1996).

Smith, S.O., Aschheim, K. and Groesbeek, M. Magic angle spinning NMR of membrane proteins. Quart. Rev. Biophys. 29, 395-449 (1996)

Han, M. and Smith, S.O. High resolution structural studies of the retinal-glu113 interaction in rhodopsin. Biophys. Chem. 56, 23-30 (1995)

Han, M. and Smith, S.O. NMR constraints on the location of the retinal chromophore in rhodopsin and bathorhodopsin. Biochemistry 34, 1425-1432 (1995) Corrections.

Arkin IT, Rothman M, Ludlam CF, Aimoto S, Engelman DM, Rothschild KJ, and Smith, S.O. Structural model of the phospholamban ion channel complex in phospholipid membranes. J. Mol. Biol. 248(4) 824-34 (1995)

Metz, G., Howard, K.P., van Liemt, W.B.S., Prestegard, J.H., Lugtenburg, J. and Smith, S.O. NMR studies of ubiquinone location in oriented model membranes: evidence for a single motionally averaged population. J. Am. Chem. Soc. 117, 564-565 (1995)

Peersen, O.B., Groesbeek, M., Aimoto, S. and Smith, S.O. Analysis of rotational resonance NMR magnetization exchange curves of crystalline peptides. J. Am. Chem. Soc. 27, 7228-7237 (1995)

Smith, S.O. and Bormann, B.J. Determination of helix-helix interactions in membranes by rotational resonance NMR. Proc. Natl. Acad. Sci. USA 92, 488-491 (1995)